Effect of Mobile Phases on the Isomeric Separation of Sirolimus in Reversed-phase Liquid Chromatography
ZHENG Quan
LI Ping
CHU Zhaowei
FAN Yubo
Abstract：The complete chromatographic separation of sirolimus isomers (β-peak and γ-peak) is helpful for accurately determining the content of sirolimus.The main purpose of this study was to examine the effect of mobile phases on the isomeric separation of sirolimus in reversed-phase liquid chromatography.Detection of sirolimus was performed using HPLC System, equipped with a YMC J'sphere ODS-H80 column.The mobile phases used were binary solvent mixtures of water/acetonitrile (40/60, 35/65, 30/70, v/v) and water/methanol (20/80, 15/85, 10/90, v/v).The experimental data showed that the retention time, peak width and resolution of sirolimus isomers (β-peak and γ-peak) all decreased significantly with the increasing organic content in the mobile phases.By adjusting the ratio of water/acetonitrile (35/65), or water/methanol (15/85), β-peak and γ-peak of sirolimus were separated to achieve good resolution.Furthermore, β-peak was observed to have a shorter retention time and narrower peak width in water/methanol (15/85, v/v) mixture than in water/acetonitrile (35/65, v/v) mixture.Consequently, these results show that rapid and high-resolution separation of sirolimus isomers is obtained in reversed-phase liquid chromatography with the mobile phase of water/methanol (15/85, v/v).
Keywords：SirolimusIsomerHigh performance liquid chromatographyIsomeric separationMobile phase
Publication Date：2017-01-01
Online Publishing Date：2026-09-11(First online date of this platform, not the publication date of the document)
Pages：4( 48-51 )