Determination of the Residual Isopropanol in Cross -linked Sodium Hyaluronate by Gas Chromatography
LI Hongmei
ZHANG Suwen
ZHU Xiaojie
GOU Xiuju
MENG Jianwen
WANG Xiaochen
ZHU Xiaomin
Abstract：A method for determination of resiual isopropanol in cross -linked sodium hyaluronate by GC chromatography was estab-lished.The method was programmed with Agilent DB -624 capillary column(30 mm ×0.535mm,3.0 μm)and FID detector.The carri-er was nitrogen and the flow rate was 2.0 ml/min,the oven introduced sequential increasing of temperature programming.The initial column temperature was 50℃ and maintained it for 2 min,then raised it to 160℃ at a rate of 10℃/min,after running temperature to 50℃,maintained it for 1 min.The calibration showed a good calibration linearity with the range of 0.01083 ~0.8664 mg/ml for isopro-panol and the correlation coefficient was 0.9990.The average recoveries were in the range of 99.1%.The relative standard deviations (RSD)was 1.2% .The method has good repeatability and high sensitivity and the method is simple and accurate.
Keywords：Cross -linked Sodium hyaluronateHeadspace gas chromatographyResidual isopropanolCrosslinkinglDetermination
Publication Date：2015-01-01
Online Publishing Date：2026-09-11(First online date of this platform, not the publication date of the document)
Pages：3( 190-191,195 )