Manufacture and Identification of Human mtDNA Genes Expressing Microarray

SUN Hengwen; HU Yide; ZENG Zijun; PAN Yi; FANG Liangyi; TAN Peixin; ZENG Xiangwei

Abstract：To manufacture and identificate the expressing microarray of human mtDNA genes.In the process of conformation,the specificity and stability of the new microarray were evaluated,the procedure of preparing and applying of the new microarray were con-summated,and the experimental parameter were optimised to provide the substance support and technology guarantee for the function research of mitochondrial genome.Design of the oligonucleotide probes of 13 mRNA、2 rRNA、9 tRNA encoded by mtDNA was based on the mtDNA Cambridge Sequence,and the oligonucleotide probes of 5 apoptosis related genes encoded by nuclear genome was based on the database of Genebank.The microarray was printed with microarray robots.Fluorescence background manifest and deletion were ac-cording to the standard process.The primary microarray was firstly used to detect the differential expressions of mtDNA genes and apop-tosis related genes between cDNA library of human cervix epithelia carcinoma cell line Hela and human renal tubule epithelia cell line Hc1.The printed spots on the primary microarray show distinct and regular on the scanned imagine,and the background of the slides is nearly undetected.The hybridized imagine of cDNA library from cell lines show a good quality in size and color contrast.Twenty mini -blocks are printed in the first round.