Application of Hough Transform for the Processing of Data for PCR -reverse Dot Blotting
WANG Gang
LI Ling
Abstract：To explore the application of Hough transform for automated interpretation of the results of polymerase chain reaction -reverse dot blotting (PCR -RDB)for the detection of genetic mutations.The Hybribio HMM-2I Rapid Hybridizer for the detection of DNA mutations was used as an example.A method was established for fast interpretation of the PCR -RDB results by image recognition using Hough transform.In addition,a set of mobile -terminals -based results processing scheme were proposed.A software was de-veloped for automatic interpretation of the PCR -RDB results for detecting common mutations in 113 thalassemia cases and subtypes of human papilloma virus in 83 cases.The results were compared with those of manual interpretation,with a consistency of 100%.The Hough transform can suit automated interpretation of the results of PCR -RDB assays.The method does not require human interven-tion,can reduce the error rate of visual interpretation,and improve the efficiency of testing.
Keywords：Polymerase chain reaction -reverse dot blottingHough transformBinary imageMedian filterGene mutation screen-ingMobile terminal
Publication Date：2015-01-01
Online Publishing Date：2026-09-11(First online date of this platform, not the publication date of the document)
Pages：7( 158-163,173 )