Quantitative Characterization and Analysis of the Shape and Distribution of Microfilaments in Rat Airway Smooth Muscle Cells
CHEN Shuyue
XU Yang
PAN Yan
LUO Mingzhi
ZENG Huilong
DENG Linhong
Abstract：Based on laser scanning confocal microscopy imaging of airway smooth muscle cells,we presented a new method to quantify and analyze the shape,orientation and their distributions of the intracellular microfilaments from the view of the cell sections. The main structure of the microfilaments were extracted by morphology and threshold segmentation method,and then quantitatively ana-lyzed by shape and orientation distribution features including number,length,width,and analyzed texture features with gray level co -occurrence matrix.The results show that the method with quantitative feature extraction can express the shape and distribution of mi-crofilaments in rat airway smooth muscle cells.Through simulated changes of actin filaments,the parameters as energy,entropy can well describe the response of filaments.
Keywords：Airway smooth muscle cellMicrofilamentCell sectionsLaser scanning confocal microscopyShape featureDistri-bution feature
Publication Date：2015-01-01
Online Publishing Date：2026-09-11(First online date of this platform, not the publication date of the document)
Pages：6( 152-157 )