Determination of Crosslinking Agent Residual in Cross-linked Sodium Hyaluronate Cell Scaffold
ZHANG Zhiyun
LIU Ronglei
CHEN Jianying
XING Yuren
WANG Qin
LING Peixue
Abstract：A method for determination of residual 1,4-butanediol diglycidyl ether(BDDE)in cross-linked sodium hyaluronate cell scaffold by GC chromatography was established.The method was programmed with Agilent HP-5 capillary column(30 m ×0.32 mm,0.25 μm)and FID detector .The carrier gas was nitrogen and the flow rate was 2.0 ml·min-1 .The oven introduced sequential increasing of temperature programming.The initial column temperature was 90 ℃,then raised it to 250 ℃at a rate of 15 ℃·min-1 , and maintained it for 8 min.The BDDE was quantified external standard.The calibration showed a good calibration linearity with the range of 0.133~1.865 μg·ml-1 for BDDE and the correlation coefficient was 0.9993.The recoveries were in the range of 96.33 %~100.57 %.The relative standard deviations (RSD)was 1.40 % (n=9).The method has good specificity,repeatability and high sensitivity.It can be used for determining the content of residual BDDE in cross-linked sodium hyaluronate scaffold.
Keywords：Cross-linked sodium hyaluronateCell scaffoldCrosslinking agent residual quantity14 -butanediol diglycidyl e-therGas chromatography
Publication Date：2015-01-01
Online Publishing Date：2026-09-11(First online date of this platform, not the publication date of the document)
Pages：4( 23-26 )