Research on Biomarkers in Rats with Cardiac Injury Induced by Isoprenaline

QI Zhenzhen; ZHANG Yingli; WANG Sanlong; WANG Chao; WANG Xue; HUANG Zhiying; WANG Jufeng; LI Bo

Abstract：This study is focused on the application value of taking cardiac troponin I(cTnI),Heart Fatty Acid-binding Protein(H-FABP)and C-reactive protein(CRP)which used as the biomarkers to diagnose preclinical cardiac injury.Male SD rats were trea-ted a single i.p.injection of isoprenaline at levels of 0.5,5 or 50 mg/kg,and were evaluated at 1,3,6,12,24 h post-dosing for testing biomarkers and cardiac histology.Cardiac troponin I(cTnI)levels was measured by luminex,Heart fatty acid-binding protein (H-FABP)and C-reactive protein(CRP)were measured by enzyme-linked immunosorbent assay(ELISA).The activities of glu-tamic-oxalacetic transaminase(AST)and creatine kinase MB(CK-MB)were determined by automatic biochemical analyzer.Rat cardiac histopathology were examined under microscope.Receiver operator characteristics (ROC)analysis was used to compare sensi-tivity and specificity of each biomakers.The mean levels of cTnI were significant elevated at 3 to 24 h in isoprenaline group.The H-FABP levels of serum were greater than that of controls at 1 to 3 h,earlier than visible cardiac injuries observed.CRP was increased at 24 h in isoprena group.In ROC analysis,the area under the curve of cTnI,H-FABP,CRP,AST and CK-MB were 0.821,0.763, 0.584,0.741,0.742,respectively.In this model,the diagnostic value of cTnI and H-FABP are higher than other classical indica-tors,while CRP showing no diagnostic value to the early cardiac injury.