Preliminary Study on the Anti-tumor Effect of HLA-A2 .1 Restricted Survivin-△Ex3 -derived CTL Epitope Gene Vaccine
CHEN Yingli
GE Cunxing
LIU Siqi
ZHANG Tingting
SUI Guangyu
CAO Mingyue
CHANG Yajuan
LIU Xinyu
Abstract：To predict and synthesize HLA-A2.1 restricted survivin-△Ex3-derived CTL epitope gene vaccine,and discuss the protective effects of oral attenuated salmonella vaccine with epitope gene on the model mice of transplanted hepatic cellular cancer. Epitope gene sequences with highest scores in prediction were connected to establish eukaryotic expression vector of pVAX1 -STEs-EGFP,which was then transferred into the attenuated salmonella to act as oral vaccine.Experimental animals were divided into the control group (un-vaccinated),oral salmonella group ,oral attenuated salmonella group (transfected by pVAX1 plasmid),oral atten-uated salmonella group (transfected by pVAX1 -Survivin -△3 (T34A)-EGFP plasmid)and oral attenuated salmonella group (transfected by pVAX1 -STEs-EGFP plasmid).The mice were given immunization and then injected liver cancer cells subcutane-ously.The size of the mass at the injection site was measured.In 5 groups of the mice,the average diameters of the tumor were respec-tively 15.11 ±2.43 mm,13.70 ±2.97 mm,13.05 ±1.77 mm,7.46 ±2.61 mm and 9.05 ±2.18 mm;Epitope gene vaccine groups have significant difference when compared with the other groups (P<0.01 ).We concluded that HLA-A2.1 restricted surviving-△Ex3-derived CTL epitope gene vaccine can inhibit the proliferation and migration of mice liver cancer cells after oral administration to mice.
Keywords：Survivin-△Ex3HLA-A2 restrictedCTL epitopeGene vaccineHepatoma carcinoma
Publication Date：2014-01-01
Online Publishing Date：2026-09-11(First online date of this platform, not the publication date of the document)
Pages：5( 120-124 )