Protective Effect of Adiponectin on Atherosclerosis by Relieve Oxidative Stress

WANG Xuemei; PU Hongwei; JIANG Tao; WEI Qin; ZHANG Chun; DUAN Mingjun; SHOU Xi; ZHANG Jianlong; YANG Yining

Abstract：To investigate the effect of different dose on atherosclerosi(AS)and oxidative stress and their dose-effect ralationship. fourty-eight healthy apolipoprotein E-deficient(apoE -/-)mice were randomly divided into four groups:control group,high-fat diet group,low and high dose of adenovirus adiponectin.12 mice for each group.The CON group were given normal chow and water.The HFD group were given high-fat diet for 8 weeks.The APN group were injected adenovirus(Ad-APN 1.0 ×108 p.f.u and 5.0 ×108 p.f.u respectly)via the tail vein every 2 weeks,last for 8 weeks.And the APN group were put on high-fat diet feeding.After animals were anesthetized,blood samples were obtained.Serum APN were measured with commercial ELISA kits.Serum superoxide dismutase (SOD)activity and malondialdehyde(MDA)was detectd by chemical chromatometry.Total cholesterol(TC),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C)levels were assessed using spectrophoto-metric asssay.RT-PCR technique was used to analyze levels of mRNA expression for endothelial nitric oxide synthase (eNOS)and Adiponectin(APN)in aortic.The lesion formation in aortic sinus was measured with histochemical analyses.Compared with HFD group,The lesion formation in aortic sinus was inhibited by 26%and 31%respectively in low-APN group and high-APN group.The serum TC,TG,HDL-C,MDA levels were decreased significantly.SOD activity were increased significantly.The mRNA expression for eNOS in aortic were increased significantly.Adiponectin may protect aortic from AS injury by reducing oxidative stress.