Effects of Cyclic Pressure on Integrin-α3β1 of Rabbit Chondrocytes

HUANG Zheng; ZHANG Zifeng; LIU Yijie; CHU Lixi; FENG Wei; CAO Yanjun

Abstract：To observe the effect of cyclic pressure on proliferation of rabbit articular chondrocytes and integrin-α3β1 production. The chondrocytes were isolated from fetal rabbit articular cartilage and cultured in vitro.Third-generation chondrocytes were plated in culture plates.We set the no intervention control group(group A),cyclicpressure group (25 PSI,group B),persistent pressure group (group C,25 PSI,sustained pressure).2 ha day of continuous pressure intervention,after 3 d,the proliferation of chondrocytes in each group were detected by the cytotoxic WST-1 cell proliferation assay kit (OD value),and ELISA assay was used to measure the culture supernatant of integrin α3 β1 levels.The OD value of group A was 1.16 ±0.08,group B was 1.52 ±0.12,and group C was 1.36 ±0.13.Comparison of OD value of each group,the difference had statistical significance (P<0.05),group A of integrinα3 β1 level was 3.64 ±0.15 pg/ml,group B was 4.03 ±0.19 pg/ml,group C was 3.77 ±0.14 pg/ml,comparison of integrinα3 β1 of each group,the difference was statistically significant (P<0.05).Cyclic pressure stimulation of rabbit chondrocytes in car-tilage can increase cell proliferation and integrin level.Cyclic pressure is more effective than continuous pressure.