Functional of a Novel Human New Gene Inducing Cell Apoptosis
LI Xiaohui
LI Xiang
TENG Wei
FANG Jian
CHEN Wenwu
Abstract：To detect the function of human new gene HDDC2 to HEK293T cell.By searching the human reference sequencing in NCBI,human functional gene HDDC2 (NM_016063)with full length 1615bp was cloned from human multi-normal tissue cDNA li-brary by using PCR method.Hddc2 protein character was analysed by bioinformatics.CCK8 assay monitorsed the cell death.FACS as-say was used to evaluate cell death and to test PS externalization.Western blot test the function of HDDC2 to human apoptosis related gene PARP.Bioinformatics showed that HDDC2 was 1615bp length,and from 205 to 819 encoding 204 amino acids with a predicted molecular mass of 23.4 kd.This gene was located on chromosome 6q22.31,and encompassed 6 exons and 5 introns,it also showed that this gene locate in mitochondria.CCK8 assay showed that HDDC2 gene could induce HEK293T cell death,and this new gene could not only induce HEK293T cell PS externalization but also disrupt mitochondrial transmembrane potential tested by Facs assay. Western blot showed that cleaved PARP was observed in HDDC2 transfected HEK293 cell.Human new gene HDDC2 is a new gene which can induce HEK293T cell apoptosis.
Keywords：New geneHDDC2Programmed cell deathFlow cytometerPoly ADP ribose polymerase (PARP)
Publication Date：2014-01-01
Online Publishing Date：2026-09-11(First online date of this platform, not the publication date of the document)
Pages：5( 24-28 )