The Role of the Signaling Factor p38MAPK on Myocardial Ischemia-Reperfusion Injury

DUAN Mingjun; CHEN Bingxin; REN Peng; LI Zhe; GUO Min; TIAN Fei

Abstract：The role of the signaling factor p38MAPK was studied on myocardial Ischemia-reperfusion injury based on the animal models.SD male rats were divided into four groups randomly (group N:sham group,group A1:ischemic 30 min and reperfusion, group A2:ischemic 60min and reperfusion,group B1:ischemic 30 min-reperfusion and Astragalus Membranaceus treatment,group B2:ischemic 60min and Astragalus Membranaceus treatment,group C1:ischemic 30min and Nitroglycerin treatment,group C2:ische-mic 60 min and Nitroglycerin).The changing of ECG in preparation and operation was inspected.After 72 of the operation,the levels of myocardial zymogram were determined and the left ventricular pressure was traced.After that,the expression about p38MAPK was observed by tocking myocardium.Myocardium zymogram (include CK、CKMB、LDH and AST)in the group A1、A2 were significantly higher than that of group N respectively.In the same ischemia times,these value in the group B、C were obviously lower than that of group A(P<0.01).While the reverse phenomenon were obtained about left ventricular pressure.The expression about p38MAPK in the group A1、A2 were significantly higher than that of group N respectively,and group B1、C1 were also significantly higher than group A1(P<0.01).This value in the group B2、C2 was only higher than that of group A2(P<0.05).Myocardial Ischemia-Reperfusion can activate p38MAPK signaling transduction path,then reduce Ischemia-reperfusion injury.