Isolated,Cultivated,Identified and Comparison of Brain and Spinal Cord Microvascular Pericytes from Wista Rats
WU Qingbin
JING Yingli
YUAN Xiaochen
LI Hongwei
XIU Ruijuan
Abstract：To compare the different characteristics of brain microvascular pericytes (BMP)and spinal cord microvacularpericytes (SCMP),they were isolated,cultured and identified.High-speed centrifugal method was used to obtain the microvassels from brain and spinal cord.Then the microvessels were cultivated in the pericyte-specific medium.Pericyte was isolated from the microvessels. NG2 and PDGFRβ were used to identify the pericytes.FITC-labeled phalloidin was used to stain F-actin.The cell cycles of BMP and SCMP were detected by flow cytometry.Western blot assay was applied to analyze the functional proteins of pericytes.The results indicated that BMPs possessed bigger cell nucleus and more unfolded cytoplasm than SCMPs.BMPs expressed much more F-actin than SCMPs (P<0.01).There was no significant difference between the two types of pericytes in cell cycle.BMP expressed more α-SMA、NG2 and PDGFRβthan SCMPs.These distinguishing features may reflect the more widespread differences between the BBB and BSCB which directly impact pathophysiological processes in various major diseases.
Keywords：Brain microvascular pericytesSpinal cord microvascular pericytesCentral nervous systemUltra-speed centrifugeFunctional proteins
Publication Date：2014-01-01
Online Publishing Date：2026-09-11(First online date of this platform, not the publication date of the document)
Pages：5( 1-5 )