The Biocompatibility Study of Regenerated Antheraea Pernyi Silk Fibroin on the Differentiation and Maturation of Murine BM -derived Dendritic Cells
LIU Qi
YU Gehua
LI Mingzhong
ZHANG Xueguang
TANG Junming
Abstract：To investigate the effect of regenerated silk fibroin on the differentiation and maturation of murine BM-derived DCs in vitro.In order to clarify the immunogenicity and biocompatibility of silk fibroin.Murine BM-derived DCs were Isolated and cultured in vitro and then transferred to the wells coated with silk fibroin.The cell morphology was observed with microscope.And the proliferation of the cells from each group was determined by MTT assay.The expressions of associated molecules were analyzed with flow cytometry. Results showed that (1)Both regenerated Antheraea pernyi silk fibroin and regenerated Bombyx mori silk fibroin could support the grow and aggregate formation of murine DCs;(2)MTT assay showed that regenerated Antheraea pernyi silk fibroin could support the proliferation of DC2.4 significantly;(3)By FCM analyses,the expression pattern of CD11c,CD40,CD80,CD86 and MHC -Ⅱmolecules on BM-derived DCs were similar between silk fibron group and blank group.Regenerated Antheraea pernyi silk fibroin can support the growth and proliferation of DC2.4 better than regenerated Bombyx mori silk fibroin did.Regenerated silk fibroin can not promote the maturation of murine BM-derived DCs.
Keywords：Silk fibroinDendritic cellsBone marrowCD moleculeFCMImmunogenicity
Publication Date：2013-01-01
Online Publishing Date：2026-09-11(First online date of this platform, not the publication date of the document)
Pages：5( 235-238,242 )