Establishment of A Tissue Culture and Rapid Propagation System for Catalpa bungei'Luqiu 3'

FU Yinyin; SONG Yanyong; HAN Youji; ZHUANG Ruonan; QIAO Yanhui; ZHONG Weiguo; LI Shanwen; JIANG He; ZHANG Chuanyu; LI Shuangyun

doi:10.3969/j.issn.1002-2724.2026.01.002

Abstract：[Objective]To solve the problems of low efficiency and high cost of traditional propagation methods for the fine variety Catalpa bungei'Luqiu 3',this study aimed to achieve efficient seedling raising by establishing its tissue culture and rapid propagation system.[Method]Taking the current-year young shoots of Catalpa bungei'Luqiu 3'as explants,orthogonal experimental design and other methods were used to determine the optimal explants and disinfection time,screen the optimal culture media for primary induction,subculture proliferation and rooting culture,and select the suitable ratio of hardening-off and transplanting substrates.Relevant growth and survival indicators were counted.[Result]Using buds germinated in hydroponics as explants,soaking in 0.10%HgCl2 for 8 minutes was the optimal treatment,with a contamination rate of 3.30%and a germination rate of 96.70%.The optimal culture conditions for each stage were screened by orthogonal experiments:the primary induction medium was MS+6-BA 0.50 mg·L-1+IBA 0.10 mg·L-1,with the number of induced buds reaching 3.60 per explant;the subculture proliferation medium was N6+6-BA 0.75 mg·L-1+NAA 0.15 mg·L-1,with a proliferation coefficient of 6.20;the rooting medium was 1/2MS+NAA 0.50 mg·L-1+IBA 0.10 mg·L-1,with a rooting rate of 92.00%and an average number of roots of 10.50 per plant.After 7 days of hardening-off,the seedlings were transplanted into the substrate with perlite:vermiculite:peat(1:1:1),and the survival rate reached 94.00%.[Conclusion]The tissue culture and rapid propagation system of Catalpa bungei'Luqiu 3'established in this study can provide technical support for its industrial seedling raising and popularization and application.