Study on the In Vitro Regeneration System of Liriodendron tulipifera
LU Jindong
LIU Yuling
WANG Yan
JI Xinyue
MU Yanjuan
SUN Tao
WANG Xinjun
LI Xiujie
LU Yizeng
ZHAO Shuzhen
Abstract：To overcome the bottleneck in asexual propagation of Liriodendron tulipifera,this study used apical and axillary buds from young shoots as explants for tissue culture.A cyclic disinfection method involving"sub culturing and disinfection procedures",was established,This method effectively addressed common challenges during the culture process,such microbial contamination,browning,leaf abscission,and excessive callus formation during bud elongation.The optimal media formulations were determined as follows:Shoot proliferation medium:1/2 MS+0.5 mg/L KT+0.1 mg·L-1 6-BA+0.1 mg·L-1 NAA.Bud elongation medium:WPM+0.1 mg·L-1 6-BA+1.0 mg·L-1 IBA+100 mg·L-1 VC.Rooting medium:WPM+0.02 mg·L-1 6-BA+1.5 mg·L-1 IBA.After 40 days of culture on the rooting medium,each shoot produced 1-4 roots,achieving a rooting rate of 73.3%.
Keywords：Liriodendron tulipiferashoot proliferationin vitro regeneration.
Publication Date：2025-12-28
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：6( 20-24,19 )