Establishment of a Rapid Propagation System of Sour jujube(Ziziphus jujuba var.spinosa)
SHI Yan
SHI Jinyan
Abstract：This study aimed to investigate the influence of different plant hormones on sour jujube stem segment differentiation,establish an*in vitro*rapid propagation system for sour jujube,and provide technical support and a theoretical basis for the production and application of tissue-cultured seedlings.To identify the optimal sterilization method for sour jujube explants,eight treatments with 0.01％HgCl a:various durations(8,10,12,16,20,6+6,8+8,10+10 min)were assessed based on contamination,browning,and survival rates.In the start-up culture,the hormone 6-BA was set at three levels of 0.5,1.0,and 1.5 mg·L-1,IBA was set at three levels of 0.1,0.2,and 0.3 mg·L-1,and NAA was set at three levels of 0.1,0.3,and 0.6 mg·L-1.The optimal hormone ratio for the start-up culture of tissue culture seedlings was screened;In subculture,the hormone 6-BA was set at three levels of 0.5,1.0,and 2.0 mg·L-1,and NAA was set at three levels of 0.0,0.2,and 0.4 mg·L-1.The effects of different concentrations of IBA(0.5,1.0,1.5 mg·L-1)and activated charcoal(0.0,0.3,0.6 g·L-1)on the rooting of tissue-cultured plantlets were analyzed.The results showed that:1.When the explants of sour jujube stem segments were sterilized with 0.01％HgCl2 for 8+8 minutes,the survival rate was the highest(83.33％).2.The optimal induction medium for adventitious buds from stem explants is MS+1.0 mg·L-1 6-BA+0.3 mg·L-1 NAA+0.1 mg·L-1 IBA+30 g·L-1 sucrose+5.8 g·L-1 agar.3.The optimal proliferation medium is MS+1.5 mg·L-1 6-BA+0.2 mg·L-1 NAA+30 g·L-1 sucrose+5.8 g·L-1.4.The optimal rooting medium is 1/2MS+1.0 mg·L-1 NAA+0.3 g·L-1 activated carbon 20 g·L-1 sucrose+5.0 g·L-1.The effect is better after dark cultivation for 7 days.5.Refining seedlings and transplanting:After rooting and cultivation,plantlets were transferred to natural light under shaded conditions.The shading level was gradually reduced until complete removal.Concurrently,the lids of the culture vessels were gradually opened until fully removed.Plants were then ready for transplanting into the field.A rapid micropropagation system for sour jujube was established,and suitable initiation medium,subculture medium,rooting medium,and rooting conditions for sour jujube were screened.The domestication and transplantation of sour jujube seedlings were successfully carried out.
Keywords：Sour jujubestem segmenttissue culturerapid propagation
Publication Date：2025-10-28
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：8( 23-30 )