Induction of Adventitious Bud Rooting and Its Endogenous Hormones in Fokienia hodginsii
YANG Dandan
WANG Xiaomei
LU Bingbing
WANG Yuxi
RONG Jundong
HE Tianyou
CHEN Liguang
ZHENG Yushan
Abstract：In order to determine the optimal hormone ratios and the effects of exogenous and endogenous hormones during the induced rooting of adventitious shoots of Fokienia hodginsii.The adventitious shoots were obtained from young and healthy stem segments of the current year sourced from Fujian cypress plants and cultured in tissue culture.The rooting rate of adventitious shoots,the contamination rate,and the changes of endogenous hormones in the plants were observed and measured under different conditioning formulas.The results showed that supplementation of 1.0 mg·L-1 IBA alone,0.5 mg·L-1 IBA+0.1 mg·L-1 NAA,or 0.5 mg·L-1 IBA+0.1 mg·L-1 IAA in combination could induce rooting of adventitic buds.The optimal auxin combination was 1.0 mg·L-1 IBA+0.1 mg·L-1 NAA,while the optimal plant growth regulator combination was 0.1 mg·L-16-BA+0.5 mg·L-1IBA.The root powder had no obvious effect on the induced rooting of adventitious buds,and direct dipping in root powder increased the contamination rate.The optimal concentration of activated carbon was 0.2g,and the optimal rooting sucrose concentration was 30g/L.During the induction of rooting,the contents of GA3,IAA and ABA were significantly altered,while the contents of cytokinins(CTK)changed little.This study has preliminarily determined the optimal hormone ratios for the rooting of F.hodginsii tissue-culture seedlings,investigated the changes in endogenous hormones,and can thus offer technical support for industrialized nursery cultivation.
Keywords：Fokienia hodginsiiodventitious budtake rootendogenous hormone
Publication Date：2025-02-28
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：9( 1-8,14 )