Study on the Factors Affecting Malus halliana Koehne Rapid Micropropagation in the Vitro Culture
Abstract：Using the stem segment of Malus halliatia Koehne as the explant, this paper probes the effects of phytohor- mone of different concentration 6--BA,KT, IBA, IAA on proliferation and regeneration of individual plant about cuvette seed-ling . The results shows that the appropriate medium for first culture is MS＋ZT0.6mg/Lq-IAA0.5mg/L ＋ sugar30g/L ＋ agar6.5g/L, the medium of multiplication is MS＋ 6 -- BA 1.5g/L-Jr- IBA 0.2mg/Lq- sugar20g/L ＋ agar6. 5g/L, the medium of rooting is 1/2MS＋IBA01.5mg/Lq-sugar2Og/L＋ agar6.5g/L, and same results is proved through orthogonal test . basic rate （1 ： 1） in the soil and vermiculite has high survival rate when one is planted after trainde seedling.
Keywords：Malus halliatia Koehnetissue cultureorthogonal tests plantletrapid propagation
Publication Date：2012-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 45-47,28 )