Isolation and Evaluation of Nerve Growth Factor
SHEN Zheng-shan
XU jing
LIU Jun
CAO Xiao-jan
LU Tian-run
Abstract：Objective: to obtain the high purified and active nerve growth factor (NGF) from mouse submaxillary glands. Methods: NGF was prepared from mouse submaxillary glands by the way of elution with CM 52 column.The molecular weight and purification of NGF were detected by SDS-PAGE polyacrylamide gel electrophoresis. The biological activity of NGF was verified thorough culturing DRG. Results: About 14 kDa stained band was observed on SDSPAGE and it promoted proliferation of dorsal root gang lia (DRG). Conclusion: Good quality of NGF could be obtained with these methods.
Keywords：nerve growth factordorsal root gangliabioactivity
Publication Date：2004-10-31
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 320-322 )