Construction of Eukaryotic Expression Plasmid pcDNA3-FHIT and Its Transient Expression in COS-1 Cells
Abstract：Objective: To construct eukaryotic expression plasmid pcDNA3-fragile histidine triad(FHIT)and obtain its transient expression in COS-1 cells. Methods: FHIT gene was cloned from normal human thyroid tissue by RT-PCR and then inserted into eukaryotic expression vector pcDNA3. After the sequence was confirmed, the recombinant plasmid pcDNA3-FHIT was transfected into COS-1 cells by cation liposome. The transient expression in the cells was measured by immunocytochemistry. Results: The sequence of FHIT in pcDNA3 was correct and high expression was obtained in COS-1 cells. Conclusion: The eukaryotic expression plasmid pcDNA3-FHIT was constructed successfully and could highly express FHIT protein in COS-1 cells. This will be potentially useful for the research on gene therapy.
Keywords：fragile histidine triadgene cloningCOS-1 celltransient expression
Publication Date：2004-08-31
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 275-277 )