Dexamethasone Induces Apoptosis of Primary Cultured Osteoblasts
Abstract：Objective: To investigate the effect of glucocorticoids on cell viability of osteoblasts and explore the cytological and molecular mechanisms of osteoblasts apoptosis induced by Glucocorticoids. Methods:Dexamethasone was used to induce apoptosis of primary cultured osteoblast-like cells from skulls of suckling mouse. Surviving rate of cells, apoptosis rate, activity of Caspase-3 and DNA binding activity of nuclear factorκB were assessed respectively. Results: It was found that Dexamethasone increased apoptosis rate of osteoblastlike cells, activated Caspase-3 while inhibited the activity of NF-κB, in a concentration-dependent and time-dependent manner. Conclusion: These results suggested that Dexamethasone induced apoptosis of primary cultured and non-transformed osteoblasts, which was Caspase-3 dependent, while NF-κB might play a protective role in the process through inhibition of Caspase-3.
Keywords：dexamethasoneosteoblast-like cellcaspases-3TNF-αNF-κB
Publication Date：2004-08-31
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 256-261 )