Expression of CyclinD1/Bcl-1 and p27/Kip1 in Gliomas:Their Correlation with Pathological Grade and Prognosis

BAI Bin; SHI Wei; LIU Wen-ke

Abstract：Objective: To study cyclinD1/bcl-1 and p27/kip1 expression in gliomas and their correlation with pathological grade and prognosis. Methods: Immunohistochemical technique was used to detect the cyclinD1/bcl-1 and p27/kip1 expression in 48 human brain glioma tissues of different malignant grades, and 12 normal non-neoplastic tissues collected from internal decompression. The data were analyzed quantitatively by the image system and also correlated retrospectively with the patients' clinical characteristics. Results: The immunohistochemical reaction for cyclinD1/bcl-1 and p27/kip1 was confined to the nuclei. The abnormal positive expression rates of both cyclinD1/bcl-1 and p27/kip1 in gliomas were found higher than that in non-neoplastic tissues( P ＜0.05). The number and staining intensity of cyclinD1/bcl-1 positive nuclei increased with malignant grades (P ＜ 0.05). On the contrary, the positive nuclei of p27/kip1 expression decreased in number and staining intensity with malignant grades( P ＜0.05). Higher expression of cyclinD1/bcl-1 or/and lower expression of p27/kip1 were associated with poor prognosis(P ＜ 0.05). Conclusion: The abnormal expression of both cyclinD1/bcl-1 and p27/kip1 might bo closely related to the occurrence and development of gliomas and they might have synergistic effect. These data suggest that both cyclinD1/bcl-1 expression and p27/kip1 expression can act as an independent prognostic factor.