Shp-2/NF-κB Pathway Mediates the Inhibition of Lipoxin A4 on IL-1β-induced Synthesis of IL-6 in Glomerular Mesangial Cells
WU Sheng-hua
LU Chao
DONG Ling
CHEN Zi-qing
Abstract：Objective: To examine whether lipoxin A4 (LXA4) has an antagonistic effect on IL-1ββ-induced synthesis of IL-6 in glomerular mesangial cells, and to explore the molecular mechanisms of signal pathway in LXA4 actions. Methods: The glomerular mesangial cells of rat were cultured and treated with IL-1β, with or without preincubation with LXA4 at different concentrations. The amount of IL-6 in the supernatant of cells was analyzed by enzymelinked immunosorbent assay(ELISA). The expressions of mRNA of IL-6 were determined by RT-PCR. The expressions of Src homology 2( SH2 ) containing protein-tyrosine phosphatase 2(Shp-2) were assessed by immunoprecipitation and immunoblotting. Activities of DNA-binding of nuclear factor-kappa B(NF-κB) were measured by electrophoretic mobility shift assay(EMSA). Results: IL-1β-stimulated secretion of protein and expression of mRNA of IL-6 in mesangial cells were inhibited by LXA4 in a dose- dependent manner. LXA4 antagonizes the phosphorylation of Shp-2 and activities of NF-κB induced by IL-1β. Conclusion: LXA4 amtagonists IL-1β-induced synthesis of IL-6 in glomerular mesangial cells through the mechanism of Shp-2/NF-κB pathway- dependent signal transduction.
Keywords：lipoxininterleukinnuclear factor-kappa BShp-2mesangial cell
Publication Date：2004-07-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 167-171 )