Growth Inhibition and Apoptosis Induced by Retinoic Acid Combined with Interferon Alpha-2a on Transitional Cell Carcinoma of Bladder
Abstract：Objective: To identify new favorable agents and develop novel approaches for the chemoprevention and treatnent of superficial bladder cancer and investigate the effects of combination of retinoids and interferon α-2a on growth inhibition and apoptosis induction in bladder cancer cell lines. Methods: Four bladder cancer cell lines, grade 1 to 3, and two retinoids, all-trans-retinoic acid ( ATRA ), 9-cis retinoic acid(9cRA ), combined with interferon α-2a ( INF ),were used in the study. We compared the competence of these agents to inhibit growth, induce apeptosis, affect the expression of nuclear retinoid receptors, and modulate STAT1 protein. Results: Most of the bladder cancer cell lines were resistant to the effect of ATRA and 9cRA on growth inhibition and apoptosis induction, even at higher concentration(10- 5M). The effects of ATRA and 9c RA on cell growth and apoptosis were enhanced by INF α-2a. Combination of ATRA and IFNα-2a induced RARβ and Stat 1 expression in three bladder cancer cell lines. Conclusion: The results demonstrated that INFα-2a synergize with the inhibitory effect of ATRA and 9c RA on the growth inhibition and apoptosis of bladder cancer cells in vitro, which suggested that it has a potential interest for the treatment of transitional cell carcinoma of bladder.
Keywords：apoptosisgrowth inhibitionretinoidsinterferonbladder cancer cell
Publication Date：2004-05-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 158-162 )