Setting up the Model of Xeno-graft Verse Host Disease
XIA GUO-Wei
ZHANG Yuan－fang
DING Qiang
Abstract：Objective: To observe human to mouse one way mixed lymphocyte(MLC); And to set up the xeno-grats verse host disease Xeno-graft host disease(XGVHD) model, probing its immunologic mechamism. ~: Mouse splenic lymphocyte were collected in asepsis and treated by mitomycin as activating cell. Human Peripheral blood lymphocytes (hPBL)were separated and gathered as reacting cell; Mouse splenic lymphocyte and hPBL were mixed to incubate for a week. Destroying recipient (mouse) immune system by total body irradiation (TBI) and intraperitoneal injecting CTX、MTX; Separating and collecting hPBL;ously, the amount of H-TdR in corporation increased evidently( P ＜ 0.05); The mean percentage of CD4、CD8、 IgG、 IgM positive sionally discovered and apoptosis cells. Conclusion:The human to mouse one-way MLC has obvious lymphocyte proliferation. By these means,we succeed in inducing XGVHD and setting up a XGVHD model.
Keywords：senograft one-waymixed lymphocyteXeno-graft host diseasetotal body irradiation
Publication Date：2004-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 32-35 )