Urocortin,the neuropeptide,inhibits the viability of ECV304 cells and rat vascular smooth muscle cells
CHEN Jie
Wang Hong-yi
Tao Jin
XU Hua-e
YANG Rong
LI Sheng-nan
Abstract：Objective:This study aims to investigate the effects of urocortin (Ucn) on the viability of endothelial cells (ECV304) and rat vascular muscle cells (VSMC). Methods: Rat aortic VSMC were isolated from the rats' thoracic aorta. We studied the effect of Ucn on the viability of ECV304 ceils and VSMC by using a tetrazolium (MTT) assay. Results: Ucn (10-7 mol/L) inhibited the viability of ECV304 cells and VSMC. Inhibition rates are 13% and 15%, respectively( P＜ 0.05, compared with Control). This inhibition was not dependent on the affecting time and was not affected by the addition of ATP-sensitive potassium channel (KATP channel) blocker, glybenclamide (Gly, 10 mol/L). Conclusion: Ucn inhibits the viability of ECV304 and VSMC.Our results suggest that Ucn may be a new vasoactive agent and may have a beneficial effect in the process of vascular remodeling(VR).
Keywords：urocortinECV304vascular smooth muscle cellsMTT assayATP-sensitive potassium channels
Publication Date：2004-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-3 )