Eukaryocytic Expression of Human B7.1 (CD80) and its Antileukemia Effect
MA Xiao-rong
ZHANG Wang-Gang
LIU Su-hu
YANG Zhang-min
CHEN Gang
Abstract：Objective: To construct the human B7. 1 (CD80) eukargocytic expressing vector and its expression on HL60 cells to investigate the immunotherapeutic and immunoprotective effects using PCR. Both of the PCR products and eukaryocytic expressing vector pHook were digested uith Apa I, Sal I and linked using T4 DNA ligase. The ligased products were transduced into DH5a.B7. 1 gene containing clones were selected by digestion with Apa I and Sal I which were furtheer con620 bp. Six clones were all digested by Apa I and Sal I to produce 620 bp gene fragment which was in the same way as B7. 1. It demonstrated that the recombinumt vector had been constructed successfulprolonged the survival time of leukemic mice, distinctively. So did the immunoprotection of B7. 1+ HL60 cells. Conclusion: The human B7. 1 (CD80) eukaryocytic expressing vector can be successfully constructed by molecular cloned methods and can be stably and availably expressed on the membrane of B7. 1 negative acute myelocytic leukemia (AML) cell line HL60. Furthermore, the costimulatory molecular vaccine has significant effection of immunotherapy and immunoprotection and gives the rationale for clinical application.
Keywords：B7.1(CD80)gene expressionimmune protection
Publication Date：2003-09-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 231-236 )