Set up Alzheimer's Disease Cell Apoptosis Model with PC-12 Cell Induced by Aβ25-35
WANG Ya-li
WANG Zhong-wei
SONG Wei
YANG Lin
Abstract：Objective: To prepare an apoptosis cell model of Alzheimer Disease (AD) by PC-12 cells treated with β-amyloid protein (Aβ). Methods: PC-12 cells were incubated with different concentrations of Aβ25-35 for different duration in vitro. The cell viability was detected by MTT assay. Morphological features of apoptosis were analyzed with Hoechst 33258/Propidium iodide dual staining, The level of intracellular free calcium ([Ca2+]i) was cakculated by Fura-2/AM fluorescence and concentration-dependent manner, and the maximal apoptosis happened at 48 h after exposure to 20 μmol/L of Aβ25-35 and 36 h to 30 μmol/L. Cell death reached the peak at 12-24 h later than the apopconcentration of Aβ25-35. The time of the highest increase rate of [Ca2+]i was about 12 h earlier than that of apoptosis. Conclusion: An AD cell model using the PC-12 cells induced with Aβ25-35 displays a series of changes related to apoptosis, which may be related to elevation of [Ca2+]i.
Keywords：β-amyloid proteinintracellular free calciumapoptosisAlzheimer dis-easecell model
Publication Date：2003-09-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 208-214 )