A Study on Antitoxic Role of Vesicular Monoamine Transporter 2 in Transgenic Chinese Hamster Ovary Cells
YE Min
DING Xin-sheng
DONG Hai-rong
QIU Zhen-ning
GUAN Xiao-hong
Abstract：Objective: To study the antitoxic role of vesicular monoamine transporter 2( VMAT2) in transgenic Chinese Hamster ovary ( CHO ) cell. Methods:With the technology of trans-gene from PC 12 to CHO, MTT reduction assay was used to detect MPP+ toxic effect on wild typeCHO ( wtCHO ) and transgenic CHO. Meanwhile, the role of reserpine was also observed in MPP+ tox-ic effects. Results:The sensitivity of transgenic CHO to MPP+ was much less than that of wtCHOwith 0. 5 mmol/L MPP+. Transgenic CHO had the same sensitivity as wtCHO if rotenone was given.WtCHO, by given reserpine alone, didn't change its sensitivity to MPP+. Conclusions : VMAT 2 hasprotective effect on transgenic CHO by transporting MPP+ to vesicles.
Keywords：transgenic Chinese Hamster OvaryVesicular Monoamine Transporter2antitoxicMPP+reserpine
Publication Date：2003-03-02
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 87-92 )