Interaction between hsa-miR-Chr8:96 and Th17 cells in dilated cardiomyopathy and its value in predicting the occurrence of heart failure

ZUKELA·Tuerhong; SHANG Shuai; GUO Yankai; JIASUOER·Xiaokereti; XU Yang; LU Yanmei; TANG Baopeng

doi:10.13431/j.cnki.immunol.j.20260004

Abstract：Objective To investigate the interaction between human microRNA chromosome 8:96(hsa-miR-Chr8:96)and helper T cell 17(Th17)in dilated cardiomyopathy(DCM)and the value of predicting the occurrence of heart failure(HF).Methods In total,153 patients with DCM admitted from September 2021 to March 2024 were selected as the observation group,and 153 healthy physical examinees in the same period were selected as the control group.The expression levels of hsa-miR-Chr8:96 in peripheral blood and the level of Th17 cells at admission or during physical examination were compared between the two groups.The interaction between hsa-miR-Chr8:96 and Th17 cells was analyzed.After standardized treatment in the observation group.The expression levels of hsa-miR-Chr8:96 and Th17 cells in the peripheral blood of patients with and without HF were observed.Multivariate logistic regression analysis was used to analyze the influencing factors of DCM complicated with HF,and smooth curve fitting was used to analyze the relationship between hsa-miR-Chr8:96,Th17 cells and DCM complicated with HF.The receiver operating characteristic(ROC)curve was used to evaluate the predictive value of hsa-miR-Chr8:96 and Th17 cells for DCM complicated with HF.Results The expression levels of hsa-miR-Chr8:96 and Th17 cells in the peripheral blood of the observation group were higher than those of the control group at admission(P<0.01).After adjusting for other factors such as gender and age,the positive additive interaction between high levels of hsa-miR-Chr8:96 and high levels of Th17 cells existed in DCM(P<0.01).After follow-up of the observation group,3 patients were lost to follow-up,and 96 patients did not develop HF,while 54 patients developed HF.The expression levels of B-type natriuretic peptide,left ventricular end-diastolic diameter,hsa-miR-Chr8:96,and Th17 cells in the observation group of patients with HF were higher than those of patients without HF at admission,and the left ventricular ejection fraction was lower than that of patients without HF(P<0.01).Multivariate logistic regression analysis showed that hsa-miR-Chr8:96 and Th17 cells were independent influencing factors for DCM complicated with HF(P<0.01).Smooth curve fitting analysis showed that after correcting for other factors,hsa-miR-Chr8:96 and Th17 cells had a positive linear relationship with DCM complicated with HF.As the levels of hsa-miR-Chr8:96 and Th17 cells increased,the risk of DCM complicated with HF gradually increased(P<0.05).ROC curve analysis showed that the area under the curve(AUC)of hsa-miR-Chr8:96 and Th17 cells for predicting DCM complicated with HF was 0.765 and 0.775,respectively.The AUC of the combined prediction of hsa-miR-Chr8:96 and Th17 cells for DCM complicated with HF was 0.884,and the combined predictive value was better than the individual prediction(Z=2.654,2.357,P=0.008,0.018).Conclusion The high levels of hsa-miR-Chr8:96 and Th17 cells in DCM have a significant synergistic effect and are independent influencing factors for DCM complicated with HF.Combined detection can significantly improve the predictive value for DCM complicated with HF.This biomarker combination is helpful for identifying high-risk patients and providing important theoretical basis and transformation direction for early intervention and individualized prevention strategies.