Diagnostic value of combined detection of serum NF-κB,G-17,and HLA-G for Helicobacter pylori infection-related early gastric cancer

WANG Jian; LIANG Lei; JIA Chunliang; LI Qingke; MA Guoming

doi:10.13431/j.cnki.immunol.j.20250128

Abstract：Objective To explore the diagnostic value of combined detection of serum nuclear transcription factor κB(NF-κB),gastrin-17(G-17),and human leukocyte antigen-G(HLA-G)for early gastric cancer related to Helicobacter pylori(Hp)infection.Methods A total of 135 patients with Hp infection admitted from February 2023 to February 2025 were selected as the research subjects.According to the results of gastric histopathological biopsy,they were divided into the control group(n=51),the precancerous lesion group(n=44),and the early gastric cancer group(n=40).The general data and serum levels of NF-κB,G-17 and HLA-G of the three groups were compared.Pearson correlation analysis was used to assess the correlations among serum levels of NF-κB,G-17,and HLA-G in patients with Hp infection,and multivariate logistic regression was used to analyze the influencing factors of early gastric cancer in patients with Hp infection.The receiver operating characteristic(ROC)curve was drawn to analyze the diagnostic value of serum NF-κB,G-17 and HLA-G for early gastric cancer related to Hp infection.Results The proportion of family history of gastric cancer and the number of neutrophils in the control group,precancerous lesion group and early gastric cancer group increased successively,while the number of lymphocytes decreased successively(P<0.05,P<0.01).The levels of serum NF-κB,G-17 and HLA-G in the control group,precancerous lesion group and early gastric cancer group increased successively(P<0.05).Pearson correlation analysis revealed that serum NF-κB was positively correlated with G-17 and HLA-G levels(r=0.522,0.481,P<0.001)in patients with Hp infection,and that serum G-17 was positively correlated with HLA-G levels(r=0.493,P<0.001).Multivariate logistic regression analysis showed that the levels of serum NF-κB,G-17,and HLA-G were risk factors for early gastric cancer in patients with Hp infection(P<0.01).The results of the ROC curve analysis showed that the areas under the ROC curve of serum NF-κB,G-17,and HLA-G detected in combination for the diagnosis of early gastric cancer in patients with Hp infection were all higher than those diagnosed by serum NF-κB,G-17 and HLA-G alone(Z=4.763,4.196,3.389,P<0.05).Conclusion The levels of serum NF-κB,G-17 and HLA-G in patients with Hp infection-related early gastric cancer increase,and the combined detection of the three has a high diagnostic value for Hp infection-related early gastric cancer.