Effect of CircPARD3 on the malignant biological behavior of acute myeloid leukemia cells via regulating R-485-5p/HOXB6 axis
BAI Yun
WANG Li
MIAO Yinsha
LIU Wei
Abstract：Objective To investigate the effects of circular RNA partitioning defective 3 homolog(CircPARD3)on the proliferation,apoptosis and invasion of acute myeloid leukemia(AML)cells via regulating the miR-485-5p/homeobox protein B6(HOXB6)axis.Methods Human bone marrow stromal cell line HS-5 and human AML cell lines AML2,U937,and HL-60 were selected.U937 cells were cultured in vitro and randomly divided into the control group,the negative control group,the miR-485-5p mimics group,the CircPARD3 siRNA group,and the CircPARD3 siRNA+miR-485-5p inhibitor group.After grouping and transfection,the mRNA and protein expressions of CircPARD3,miR-485-5p and HOXB6 in each group of cells were determined by RT-qPCR and Western blot.The apoptosis,proliferation,migration and invasion of cells in each group were detected by flow cytometry,Edu staining,cell scratch and Transwell assay,respectively.Cell apoptosis and the expression of proteins related to epithelial-mesenchymal transition were detected by Western blot.The targeted regulatory effects of CircPARD3 on miR-485-5p and miR-485-5p on HOXB6 in U937 cells were analyzed by dual-luciferase reporter assay.Results The mRNA and protein expressions of CircPARD3 and HOXB6 in U937,AML2 and HL-60 cells were all increased compared with those in HS-5 cells(P<0.05),while the expressions of miR-485-5p were lower than in those in HS-5 cells(P<0.05).Compared with the control group,the mRNA and protein expressions of HOXB6,proliferation rate,migration number,invasion number,and Vimentin protein expression in the CircPARD3 siRNA group and the miR-485-5p mimics group were all decreased(P<0.05).The expressions of miR-485-5p,the apoptosis rate,and the protein expressions of Bax,Cleaved Caspase-3,and E-cadherin were all increased(P<0.05).Compared with the CircPARD3 siRNA group,the mRNA and protein expressions of HOXB6,proliferation rate,migration number,invasion number,and Vimentin protein expression in the CircPARD3 siRNA+miR-485-5p inhibitor group were all increased(P<0.05),while the expressions of miR-485-5p,the apoptosis rate,and the protein expressions of Bax,Cleaved Caspase-3,and E-cadherin were all decreased(P<0.05).CircPARD3 could target and down-regulate the expression of miR-485-5p in U937 cells,and miR-485-5p could target and down-regulate the expression of HOXB6.Conclusion Knockdown of CircPARD3 expression can induce apoptosis of AML cells and inhibit their proliferation and invasion by regulating the miR-485-5p/HOXB6 axis.
Keywords：acute myeloid leukemiacircular RNA partitioning defective 3 homologmiR-485-5p/homeobox protein B6 axiscell proliferationcell apoptosiscell invasion
Publication Date：2025-12-28
Online Publishing Date：2026-01-13(First online date of this platform, not the publication date of the document)
Pages：9( 849-857 )