The correlation of cold-induced RNA-binding protein and NF-κB levels with cognitive dysfunction in elderly patients with ischemic cerebral small vessel disease

CUI Luyan; ZHAO Xinfeng

doi:10.13431/j.cnki.immunol.j.20250050

Abstract：Objective To explore the correlation between cold-induced RNA binding-protein(CIRP)and NF-κB levels with cognitive dysfunction in elderly patients with ischemic cerebral small vessel disease.Methods 135 elderly patients with ischemic cerebral small vessel disease admitted to our hospital from March 2021 to June 2024 were selected as research subjects.They were divided into a cognitive normal group(n=48)and a cognitive impairment group(n=87)based on whether cognitive dysfunction occurred.Multiple linear regression was used to analyze the relationship between cognitive impairment and inflammatory indicators,while the dose-response relationship of CIRP and NF-κB with cognitive impairment were estimated using restricted cubic spline method.The cognitive impairment group was further divided into mild cognitive impairment group(n=30),moderate cognitive impairment group(n=32),and severe cognitive impairment group(n=25)based on the severity of the condition.Ordered regression analysis was used to investigate the impact of CIRP and NF-κB on patient's condition;ROC curve was used to evaluate the predictive value of CIRP and NF-κB for moderate to severe cognitive impairment,and explore the mediating role of inflammation related factors between CIRP/NF-κB and mini-mental state examination(MMSE)scores.Results TNF-α,IL-6,IL-1β,CIRP and NF-κB are all factors affecting cognitive impairment(P＜0.05).CIRP and NF-κB were significantly positively correlated with TNF-α,IL-4,IL-10,IL-1β,MCP-1,TGF-β1 and hs CRP(P＜0.05),and significantly negatively correlated with TGF-β1(P＜0.05).The correlation of CIRP and NF-κB levels with cognitive impairment showed a non-linear dose-response relationship(P＜0.05).There was still an independent correlation(P＜0.05)of TNF-α,IL-6,IL-1β,CIRP and NF-κB with the severity of cognitive impairment.High levels of CIRP and NF-κB showed a synergistic effect with high levels of TNF-α,IL-6 and IL-1 β.The AUC value of CIRP and NF-κB for predicting severe cognitive impairment were 0.745 and 0.792,respectively,with a combined prediction AUC of 0.840.TNF-α,IL-4,IL-10,IL-1β,MCP-1,TGF-β1 and hs CRP play a mediating regulatory role between MMSE score and CIRP/NF-κB.Conclusion CIRP and NF-κB participate in the regulation of inflammatory responses and are closely related to cognitive dysfunction and severity of elderly patients with ischemic cerebrovascular disease.Monitoring the changes of CIRP and NF-κB are beneficial for clinical evaluation of patients' cognitive function.