Mechanism of electro-acupuncture at jiaji acupoint in regulating TREM2-mediated microglia activation to promote spinal cord injury repair
JIANG Shan
WU Chao
MA Ling
Abstract：Objective To investigate the mechanism of neuronal apoptosis in rats with spinal cord injury through the activation of microglia regulated by TREM2 by electro-acupuncture at jiaji acupoint.Methods The Basso-Beattie-Bresnahan and ramp test were used to assess movement function and balance function in rats.TUNEL assay for neuronal apoptosis in spinal cord tissue of rats in each group.Positive expression of TREM2 in microglia of spinal cord tissue detected by immunofluorescence.Western blot detection of protein expression of Iba-1,IL-6,iNOS and Arg-1 in spinal cord tissue.ELISA for the determination of inflammatory factors TNF-α and IL-1β in rat spinal cord tissue.Results Compared with the SCI group,the BBB score and slope angle were significantly higher in rats treated with chiropractic electroacupuncture,and movement function and balance function were improved in SCI rats.At the same time,positive expression of TREM2 and protein expression of Iba-1,IL-6 and iNOS were reduced,Arg-1 was increased,the number of neuronal apoptosis was significantly reduced(P<0.05).In addition,administration of TREM2 activator intervention resulted in a diminished therapeutic effect of entrapment electrodes on SCI.Conclusion The electroacupuncture at Jiaji Acupoints treatment may inhibit the apoptosis of neuronal cells in the spinal cord tissue through the activation of microglia regulated by TREM2,improving SCI spinal cord injury and restoring movement function.
Keywords：Electroacupuncture at jiaji acupointsTREM2Microglia activationNeuronal apoptosis
Publication Date：2024-10-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 761-766 )