Effects of sufentanil on IL-1β-induced chondrocyte damage by regulating the JAK2/STAT3 signaling pathway

QI Xinfeng; HUANG Qingxian

doi:10.13431/j.cnki.immunol.j.20240097

Abstract：Objective To investigate the effect of sufentanil(SUF)on interleukin-1β(IL-1β)-induced chondrocyte damage and its regulatory relationship on the Janus activated kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)signaling pathway.Methods Rat chondrocytes were randomly separated into control group(NC group),IL-1β group,SUF low concentration(SUF-L)+IL-1β group(SUF-L+IL-1β group),SUF high concentration(SUF-H)+IL-1β group(SUF-H+IL-1β group),and SUF-H+IL-1β+STAT3 activator Colivelin group(SUF-H+IL-1β+Colivelin group).CCK-8 method and clonal formation assay were applied to detect the proliferation of chondrocytes,while flow cytometry was used to detect the apoptosis;qRT-PCR method was applied to detect the relative mRNA expression levels of JAK2 and STAT3;Western blot was used to detect the expression level of JAK2/STAT3 signaling pathway related proteins;ELISA was applied to determine the expression levels of IFN-γ,IL-6 and IL-8 in cell culture media of each group.Results Compared with the NC group,the cell survival rate and the clones formation number of the IL-1β group decreased,while the apoptosis rate,relative expression levels of JAK2 and STAT3 mRNA,the values of p-JAK2/JAK2 and p-STAT3/STAT3,and the expression levels of IFN-γ,IL-6 and IL-8 in the cell culture medium all increased(P<0.05);compared with the IL-1β group,the changes in corresponding indicators of chondrocytes in the SUF-L+IL-1β group and SUF-H+IL-1β group were opposite to the above(P<0.05);Colivelin reversed the protective effects of high-dose SUF on IL-1β-induced chondrocyte damage(P<0.05).Conclusion SUF can protect chondrocytes from IL-1β-induced damage by down-regulating the JAK2/STAT3 signaling pathway.