Transcriptomics-based screening of key genes for pyroptosis in macrophages regulated by mTORC1 signaling
HAO Xiyan
ZHANG Meng
YAO Ruiyuan
Abstract：Objective To explore the mechanisms of macrophage pyroptosis regulated by mTORC1 signaling based on transcriptomic sequencing.Methods Western blot,immunofluorescence and ELISA were used to detect the expression of pyroptosis-related proteins in macrophages before and after treatment of mTORC1 specific inhibitor rapamycin.In addition,the differentially expressed genes and proteins associated with mTORC1 signaling were screened by transcriptome analysis.Results mTORC1 inhibition caused macrophage pyroptosis.After rapamycin treatment,transcriptomic sequencing identified a total of 206 differentially expressed genes(DEGs),including 90 upregulated and 116 downregulated ones.The GO function annotation and KEGG enrichment analysis of the DEGs further screen out 4 DEGs(NLRC4,NOTCH4,CARD10,EBF4),which were consistent with the expression trend of transcriptome sequencing.Conclusion This study confirmed that NLRC4,NOTCH4,CARD10 and EBF4 are the key genes for macrophage pyroptosis regulated by mTORC1 signaling,constituting a novel mechanism of macrophage pyroptosis and laying a theoretical foundation for the prevention and treatment of the diseases related to pyroptosis.
Keywords：PyroptosisKey genesmTORC1Transcriptome
Publication Date：2024-09-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 694-701 )