Effects of interfering the expression of IL-8 on biological characteristics of breast cancer cells

MOU Chengjin; PAN Wu; LI Juan; WANG Jing

doi:10.13431/j.cnki.immunol.j.20180118

Abstract：Interleukin-8 (IL-8) was closely related to the occurrence and development of tumors, but its specific role in breast cancer cells is not fully understood. To investigate the effects of interleukin-8 (IL-8) on the biological characteristics of breast cancer MDA-MB-231 cells and its mechanism. The siRNA IL-8 was transfected into breast cancer cells. The experiment were divided into blank control group, negative control group, siRNA1 IL-8 group, siRNA2 IL-8 group. The viability ofbreast cancer cells was measured by MTT assay. The invasion and migrationwere analyzed bytranswell assay. The levels of IL-8 were examined by real-time RT-PCR and ELISA. The activity of Wnt/β-catenin signal pathway were detected by Western blot. Compared with the blankcontrol group, the expression of IL-8 mRNA and protein in breast cancer cells in group siRNA1 IL-8 and siRNA2 IL-8 were decreased significantly (P＜0.05). The levels of IL-8 mRNA and protein in siRNA2 IL-8 group were significantly lower than those in siRNA1 IL-8 group (P＜0.05), indicating that siRNA2 IL-8 had strong interference effect, so it will be used for the subsequent experiments. The expression of interfering the expression of IL-8 had no significant effect on the viability of breast cancer cells, but inhibited invasion and migration ability of breast cancer cells (P＜0.05), reduced the protein levels of β-catenin, MMP-2, MMP-9 (P＜0.05). Interfering the expression of IL-8 may inhibitthe invasion and migration of breast cancer MDAMB-231 cells by inhibiting the Wnt/β-catenin signaling pathway.