Establishment of fluorescence immunochromatography for detection of HPV16/18 E6 protein
SHI Yingjie
WANG Yunlong
MING Liang
LI Yulin
WANG Jichuang
PAN Weicheng
Abstract：This study aimed to establish a method of fluorescence immunochromatography for quantitative detection of human papillomavirus (HPV) 16/18 E6 protein in human cervical epithelial cells. Fluorescence immunochromatographic assay and double antibody sandwich method were used to prepare fluorescence immunoassay test strip, and the linear range, precision and specificity were evaluated. The quantitative detection of HPV16/18 E6 protein was achieved. The linear range of the method for HPV16 E6 protein detection was 2. 0-200 ng/ml and the linear range for HPV18 E6 protein was 2. 0-200 ng/ml. There was no significant cross-reaction with SCCa and HE4 in the detection processes. The intra-assay CV was less than 10％, the inter-assay CV was less than 15％, and the average recovery rates were 102. 4％ and 98. 1％ respectively. The sensitivity of this method were 91. 3％, 84. 2％ and the specificity up to 100％ in detecting 46 cervical cancer samples, 38 precancerous lesions CIN Ⅲsamples and 52 healthy samples. In conclusion, the fluorescence immunochromatographic assay for the quantitative detection of HPV16/18 E6 protein has been preliminarily established, which provides a new detection method for clinical screening of cervical cancer.
Keywords：Human papillomavirus type 16/18 E6 proteinFluorescent microspheresImmunochromatographyJoint detection
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 534-538,546 )