Effects of IL-31RA gene knockout on the airway inflammation in mouse asthma model
JIANG Tao
GAO Jin
YUE Huan
GAO Yaqian
HUANG Junqiong
Abstract：To investigate the effect of IL-31 receptor signaling on airway inflammation of mouse asthma model, an allergic asthmatic mouse model was induced by sensitizing and challenging with ovalbumin (OVA). Within 24 hours after last stimulating, the lung tissues were collected for HE staining and toluidine blue staining. The bronchoalveolar lavage fluid (BALF) were collected for WBC and EOS counting, while, the levels of Ig E, IFN-γand IL-4 in plasma were detected by ELISA. Data showed that the IL-31 RA gene knockout homozygous mouse model and asthma mouse model had been established successfully. There were great increasing of total cells and eosinophils in the BALF of asthmatic groups; the levels of Ig E in KO-OVA group were significantly elevated, as compared with control group, and were more significant higher compared with WT-OVA group. The inflammatory cell infiltration in BALF and lungs increased in KO-OVA group mice compared with WT-OVA group mice. Compared with the mice of control group, asthma mice displayed higher Th2 type cytokine level of IL-4, but lower Th1 cytokine level of IFN-γ in plasma. Furthermore, the level of IL-4 in KO-OVA was significantly higher than that in WT-OVA group. Taken together, IL-31 receptor A signaling inhibits airway inflammation in asthma mice.
Keywords：Interleukin-31 recptor AGene knockoutAsthmaAirway inflammation
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 487-491 )