Effects of erythropoietin on amyloid-β-induced microglia activation
JIANG Juntao
ZHANG Zhiren
WU Yuzhang
Abstract：This study was designed to investigate the effects of erythropoietin (EPO) signaling pathway on microglia function and to provide a theoretical basis for the therapeutic effect of EPO on Alzheimer disease (AD). BV2 microglial cells were treated with Aβ) (1-42), FITC-Aβ1-42 and rh EPO. Then the expression of inflammatory cytokines m RNA were detected by real-time fluorescence quantitative PCR, cell survival was detected by CCK-8 assay, and the phagocytosis of FITC-Aβ1-42 was detected by flow cytometry. Data showed Aβ1-42 treatment resulted in elevation of TNF-α, IL-1β, IL-6 and IL-10 m RNA levels in BV2 cells, and EPO intervention inhibited the pro-inflammatory cytokines TNF-α, IL-1β and IL-6 m RNA levels, but increased m RNA levels of anti-inflammatory cytokine IL-10. Aβ1-42 could inhibit the survival of BV2 cells, while EPO could improve the survival of BV2 cells treated with Aβ1-42; EPO could promote the phagocytosis of FITC-Aβ1-42 by BV2 cells. In together, EPO can improve the survival of microglia, promote its phagocytosis of Aβ1-42, and inhibit inflammatory activation, which is of potential significance for the study of pathogenesis, prevention, and treatment in AD.
Keywords：ErythropoietinAlzheimer's diseaseMicrogliaInflammation
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 482-486 )