Immunophenotyping analysis and its clinical significance in Kawasaki disease

CHEN Xuemei; CHEN Xin; LIU Dawei; SONG Xuerui; AN Yunfei; ZHAO Xiaodong; ZHOU Li'na

doi:10.13431/j.cnki.immunol.j.20180019

Abstract：To analyze the quantitative alternation of lymphocyte subsets in acute period Kawasaki disease (KD) and its significance in clinical diagnosis and treatment,we collect and prospectively analyzed the clinical data of 20 patients with acute KD,who were treated in Children's Hospital of Chongqing Medical University,with 20 healthy children as control group.The levels of lymphocyte subsets (CD3+,CD4+ and CD4+ subsets,CD8+ and CD8+ subsets,TCR αβ+DNT,TCR γδ+,CD4/CDS,CD19+ and CD19+ subsets,CD16+CD56) were compared between two groups;the relationship between clinical indicators and lymphocyte subsets was analyzed simultaneously.Data showed that compared with the control group,the levels of CD8 naive,TCR αβ + DNT,CD4/CD8,CD19 + and CD19+ subsets were increased (P＜0.05) in KD group,while the levels of CD4 EM (CD4 + CD45RA-effector memory T cells),CD4 Temra (CD4+ CD45RA+ effector memory T cells),CD8+T,CD8 CM (CD8+ CD45RA-central memory T cells),CD8 Temra (CD8+ CD45RA+ effector memory T cells),CD16+CD56 were decreased (P＜0.05).Inaddition,the level of TCR γδ+T cells was increased (P＜ 0.05) and CD4 Temra cells had a downward trend in KD patients with coronary artery lesions (CAL),as compared with patients without CAL;the levels of CD3 + T,CD8 CM,CD8 EM,CD8 Temra cells were decreased (P＜0.05),while the transitional B cells was increased (P＜0.05) in KD patients with complication of respiration system or digestive system,as compared to those of nRS or nDS patients.Taken together,the function of T and B cells in patients with acute KD is abnormally activated;the TCR γδ +T cells and CD4 Temra are related to the KD with coronary artery lesions,while the CD3+ T,CD8 CM,CD8 EM,CD8 Temra,transitional B cells are related to the KD with complication of respiration system or digestive system.