The mechanism of T follicular helper subsets abnormality in children with Henoch-Sch(o)nlein purpura
TANG Xiaoxiao
LI Ting
CHEN Xuelan
ZHANG Gaofu
LI Xiaoqin
WANG Mo
YANG Haiping
LI Qiu
Abstract：The aim of this study was to examine the frequency of different subsets of T follicular helper (Tfh) cells in children with Henoch-Sch(o)nlein purpura (HSP),and explore the role of Tfh in HSP.Total of 45 children with HSP were classified into three groups as followed:21 HSP children without renal involvement (group A),12 HSP nephritis (HSPN) children with only microhematuria (group B),12 HSPN children with proteinuria (group C),while 17 healthy children were recruited as controls (HC).Flow cytometry was employed to detect the percentage of Tfh (CD3+CD4+CXCR5+) cells,Tfh1 (CCR6-CXCR3+Tfh),Tfh2 (CCR6-CXCR3-Tfh),Tfh17 (CCR6+CXCR3-Ffh),ICOS,and OX40 in peripheral blood from the patients.Data showed that compared with control,the percentage of circulating Tfh cells were significantly higher in group A and C (P＜0.05),Tfh2 cells,the ratio of Tfh2+Tfh17 to Tfh1,the MFI of ICOS in Tfh cells and Tfh2 cells,OX40+Tfh cells were significantly increased in group C,MFI of ICOS in Tfh cell was increased in group A,while there were no significant difference in the percentage of Tfh1,Tfh2,Tfh17 and the expression of ICOS and OX40 in Tfh subsets between group A and B (P＞ 0.05).Moreover,the percentage Tfh cells,Tfh2 cells,MFI of ICOS in Tfh and OX40+Tfh were positively correlated with serum IgA,while Tfh1 cells were negatively correlated with serum IgA.In conclusion,increased circulating Tfh cells and imbalanced Tfh subsets play a significant role in development of Henoch-Sch(o)nlein purpura (HSP).Overexpression of ICOS and OX40 may be a underlying mechanism of abnormal activation of Tfh.
Keywords：Henoch-Sch(o)nlein purpuraT follicular helper cellICOSOX40
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 120-127 )