Protective effects of A20 on human umbilical vein endothelial cell under LPS-induced inflammatory state and its mechanism
CHEN Hongwei
ZHOU Shu
LI Xinwei
Abstract：To investigate the effect of A20 on human umbilical vein endothelial cell (HUVEC) under LPS-induced inflammatory state and its underlying mechanisms,the apoptosis of HUVEC after LPS stimulation was detected by CCK-8 and flow cytometry methods,and the tube forming experiment was observed under the microscope.The activation of NF-kappa B pathway and the secretion of soluble E-selectin in supernatant were detected by Western blotting and ELISA,respectively.Data showed that A20 inhibited the apoptosis rate of LPS-stimulated HUVEC (P＜0.001),as compared to control.Moreover,A20 promoted the rate of tube forming of LPS-stimulated HUVEC (P＜0.001),and protected the HUVEC from inflammation by inhibiting the NF-kappa B pathway.In conclusion,A20 can reduce E-selectin expression by inhibiting NF-kappa B pathway under the condition of LPS-induced inflammation,thus protects the HUVEC activity and function from inflammatory stimulation,suggesting A20 can be considered as a biomarker and target in cardiovascular diseases treatment.
Keywords：A20InflammationNF-kappa BHuman umbilical vein endothelial cell
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 115-119 )