Effects of Cremanthodium humile total terpenes on Th1/Th2 drift and Bax/Bcl-2 balance in H22 tumor-bearing mice
WANG Ruiqiong
GUO Chao
WANG Zhiwang
LIU Dongling
NIU Tinghui
WU Guotai
Abstract：This study was performed to investigate the influence of total terpenes extract of Cremanthodium humile Maxim (TTC) on Th1/Th2 and Bax/Bcl-2 balance in H22 tumor-bearing mice.The mouse models of H22 liver cancer were established by inoculation of H22 ascites.And then the general condition and the body weight change of mice were observed,the tumor inhibition rate and organ index of each group were calculated.Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of Th1 (IL-2,IFN-γ) and Th2 (IL-4,IL-10) in serum and spleen of mice;immunohistochemical method was used to detect the expression of Bax and Bcl-2 proteins in tumor tissues.The pathological changes of tumor and liver tissue were observed by HE staining.Data showed that 100-400 mg/kg TTC could significantly improve the general condition and inhibite tumor growth in H22 tumor-bearing mice.The level of IL-2 in serum and spleen were increased,but the levels of IFN-γ,IL-4,IL-10,IFN-γ/IL-4 in serum and spleen were decreased in TTC treated mice,while Th1/Th2 drift state was inhibited by TTC.Immunohistochemical staining showed that TTC could down-regulate the expression of Bcl-2 and up-regulate the expression of Bax,while HE staining showed that TTC could improve the pathological changes of tumor and liver tissues.In conclusion,TTC can inhibit tumor growth,and the mechanism may relate to inhibiting the Th1/Th2 drift state,and increasing the ratio of Bax/Bcl-2 in tumor tissue.
Keywords：Cremanthodium humileTotal terpeneTh1/Th2 driftBax/Bcl-2
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 101-108 )