Inhibitory effects of baicalin on NLRP3 inflammasome activation and pyroptosis and the underlying mechanism
YAN Liang
LI Chenguang
XU Lihui
JING Yanyun
BAI Wenjing
OUYANG Dongyun
HE Xianhui
Abstract：This study was performed to explore the influences of baicalin on ATP-induced inflammasome activation and pyroptosis in LPS-primed murine macrophages (J774A.1 or peritoneal macrophages) and the underlying mechanism.Peritoneal macrophages were elicited by intraperitoneal injection of 30 g· L-1 thioglycollate medium into C57BL/6 mice;ATP was used to activate the NLRP3 inflammasome in LPS-primed macrophages.The protein levels of IL-1 β,caspase-1 and HMGB 1 expressed in cell lysates and culture supernatants were evaluated by Western blotting;soluble IL-1β in the culture supernatants was determined by bead-based immunoassay (CBA).Influences of baicalin on pyroptosis induced by LPS plus ATP were analyzed by propidium iodide (PI) staining.Data showed that baicalin dose-dependently inhibited caspase-1 activation,mature IL-1β and HMGB1 secretion in the LPS-primed macrophages upon ATP stimulation.Baicalin also suppressed pyroptosis in murine macrophages stimulated with LPS plus ATP.Adenyl cyclase (AC) inhibitor MDL12330A or protein kinase A (PKA) inhibitor H89 could significantly reverse baicalin-mediated suppression on ATP-induced pyroptosis.In conclusion,baicalin suppresses NLRP3 in flammasome activation and pyroptosis,probably through modulating PKA activity in macrophages.
Keywords：BaicalinInflammasomePyroptosisMacrophagesProtein kinase A
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 93-100,114 )