The sphingosine 1-phosphate receptor agonist FTY720 interferes the distribution of DC and induces the maternal fetal immune tolerance
XIONG Miao
XU Liang
LI Li
LIU Yang
ZHOU Fangfang
HUANG Hongling
ZHU Jieping
Abstract：To investigate the induction mechanism of FTY720,an agonist of the sphingosine 1-phos-phate (S1P) receptor,in the maternal fetal immune tolerance,the effect of adoptive transferring of FTY720 treated DCs on embryo loss rate was calculated.Immunohistochemistry and flow cytometric analysis were employed to analyze the apoptosis and migration of DCs and the expression of CCR7 on DCs,Chemotaxis assay was performed to verify the effect of FTY720 on chemotaxis of DCs.Data showed that FTY720 had no significant effect on the embryo loss rate in normal pregnant rats.In contrast,adoptive transferring of FTY720 could significantly reduce the embryo loss rate of the spontaneous abortion mouse model (P＜0.05).DCs from blood and bone marrow were all expressed S1PRs,while FTY720 administration down-regulated CCR7 expression on DC,thus reduced transendothelial migration of DC to CCL19 (P＜0.05).FTY720 did not interfere with DC differentiation or affect the apoptotic death of DC (P＞ 0.05).We concluded that FTY720 induces maternal fetal immune tolerance through down-regulation of CCR7 expression on DC,which reduces the migration of DCs to maternal-fetal interface tissue.
Keywords：Chemokine receptor 7Chemokine ligand 19Maternal fetal immune toleranceFTY720S1P receptor
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 41-47 )