Elastase exacerbates inflammatory response and tissue damage in acute otitis media
MA Yurong
FAN Fangmei
WANG Zimeng
WANG Wei
DONG Yilin
HE Qian
ZHANG Xinxin
HE Yujuan
Abstract：To study the role of neutrophil elastase (NE) in the sterilization process and tissue injury,we developed a mouse model of acute otitis media (AOM) by direct transtympanic inoculation with Streptococcus pneumoniae (S.pn) and intraperitoneal injection of NE specific inhibitors.We collected the middle ear tissues for histological examination and the middle ear lavage fluid (MELF) for NE enzyme activity detection,S.pn load determination,inflammatory cells quantification and cytokines measurement at day 1,3,5 after inoculation,respectively.Data showed that the activity of NE in MELF supernatant significantly increased and peaked at day 3 in S.pn infected mice,as compared with the PBS group.The levels of TP,LDH,TNF-α and IL-1β3 in MELF decreased after Sivelestat treatment,a NE specific inhibitor,while the S.pn load had no significant difference compared with control group.In summary,our study demonstrats that NE can enhance inflammatory response and aggravate tissue damage,but do not involve in bacterial clearance during S.pn AOM.
Keywords：Acute otitis mediaS.pneumoniaeNeutrophilsElastase
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 34-40 )