The difference and significance of the function and frequencies of CD4+ Tregs and CD8+ Tregs in humanized NOD mice and NOD mice
ZHANG Mengjun
HAN Qingjuan
CHEN Xiaoling
CHEN Yutong
WANG Li
Abstract：In order to reveal the difference of splenic CD4+ Tregs and CD8+ Tregs in the diabetes development between humanized NOD mice and NOD mice,we observed the frequencies of CD8+CD122+T,CD8+CD28-T,CD8+ CD25+Foxp3+ T and CD4+CD25+Foxp3+ T cells in spleen and pancreatic lymph nodes from humanized NOD mice and NOD mice.The frequencies of CD4+ Tregs and CD8+ Tregs were evaluated by flow cytometry.Data showed that there was no significant difference in the frequency of CD4+CD25+Foxp3+ T cells in spleen and pancreatic lymph nodes between humanized NOD mice and NOD mice.Whereas the frequencies of the CD8+CD122+ T,CD8+CD28-T,and CDS+CD25+Foxp3+ T cells in spleen and pancreatic lymph nodes from humanized NOD mice were significantly lower than those of NOD mice.Furthermore,splenic CD4+CD25+ T and CD8+CD25+ T cells from both humanized NOD mice and NOD mice significantly inhibited the proliferation of their CD4+CD25-T cells.Our experiment result indicated that the lower frequencies of CD8+ Tregs in spleen and pancreatic lymph nodes and the higher frequencies of CD8 + T cells in pancreatic lymph nodes of humanized NOD mice might be associated with acceleration of type 1 diabetes in humanized NOD mice.These results would be helpful to the studies of clinical transformation for the prevention of type 1 diabetes.
Keywords：Type 1 diabetesHumanized NOD miceCD4+ TregsCD8+ Tregs
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 19-26 )