Effects of Mycobacterium tuberculosis Rv2986c on dendritic cells maturation and function
SUN Yan
WANG Xinqian
WU Qihang
ZHAO Longqi
LI Haibo
ZHAN Weihong
ZHANG Shulin
YU Xiaoli
Abstract：This study was designed to investigate whether Rv2986c,a newly identified antigen of Mycobacterium tuberculosis,can mature mouse dendritic cells and induce polarization of na1ve CD4 + T cells.The Rv2986c was expressed and purified,and used to stimulate dendritic cells.Then flow cytometry was used to detect the expression level of MHC-Ⅱ surface molecules on the dendritic cells,while ELISA was used to test the secretion levels of IL-6 and IL-12p40 in culture supernatant.In addition,the stimulated dendritic cells was co-cultured with na(i)ve CD4+ T cells,and the secretion level of IFN-γ in the supernatant was tested by ELISA.Data showed that the expression level of MHC-Ⅱ increased significantly on Rv2986c-stimulated dendritic cells.Moreover,ELISA showed that the secretion of IL-6,IL-12 p40 and IFN-γ enhanced obviously after Rv2986c stimulation.In conclusion,Rv2986c can activate mouse dendritic cells to present antigen and contributes to Th1 type polarization of na(i)ve CD4+T cell.
Keywords：Mycobacterium tuberculosisRv2986cDendritic cellsTh1 type polarization
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1028-1033 )